An entire paper lab book appended to a PLoS ONE paper!
Gregory I. Lang and David Botstein recently published a paper (A Test of the Coordinated Expression Hypothesis for the Origin and Maintenance of the GAL Cluster in Yeast) which is attracting attention because the supplementary data consists of a 101 page lab book containing all the notes, strain construction, methods and raw data that went into producing the paper. As Mark Hahnel pointed out in SciCrunch, this is an admirable example of both open science and thorough science. But as Mark and several people who commented on Mark’s post noted, the authors would have (a) saved themselves a lot of time and (b) made it easier for themselves and others to make use of the data they generated if they had recorded their data electronically.
To bring this point home, I thought it would be interesting to illustrate it in a very concrete way, by showing how the data from the paper could look, and be integrated with other parts of the research, like protocols, constructs, and strains, when gathered and presented in an electronic lab notebook. First, here’s how it looks using the paper approach.
The paper lab book view
The paper itself follows a standard format with the following headings:
- Abstract
- Introduction
- Results
- Discussion
- Materials and Methods
- Supporting Information
- Acknowledgements
- Author Contributions
- References

And the paper lab book also follows a familiar format, with dated entries. The entries contain the narrative of what was done, thoughts about results that were emerging, calculations, formulas, materials and constructs used in the experiment, etc. Here’s the first entry

Headings mirror publication format
Using an electronic lab notebook would have given the authors a big win right at the outset, because the electronic lab notebook can be set up with the same headings that will be used in the paper where the experimental results are presented and discussed, i.e. Abstract, Introduction, Results, Discussion, Materials and Method, Supporting Information, Acknowledgements, Author Contributions, References.
To illustrate this point, here’s how the blank template looks in the eCAT electronic lab notebook

So the data for each section of the paper is already organized and available for selective export into the document used for publication.
Presentation stays the same
Here’s what the Notebook field looks like with the initial entry filled in – just like it does in the paper notebook.

Flexible set up
There is plenty of flexibility in formatting the form used to document experiments. In this example it’s been set up so that the fields exactly follow the sections in the PLOS ONE publication, and one additional field called Notebook has been added at the top. That’s for recording the daily narrative as in the paper notebook. But the form can be set up with any fields – e.g. as follows where certain fields — Abstract, Introduction, Acknowledgements, etc. — have been removed to simplify the experiment form.

An entire working environment
With the electronic lab notebook the notebook is not isolated from the other parts of the research. In addition to experiments, protocols, constructs, etc. can also be created in the notebook. For example, here’s a protocol

Linking
A big benefit of the electronic lab notebook over paper is the ability to make links. So for example in the Materials and Methods section of the experiment, a link could be added to the protocol used in the experiment, as shown below

Now, whenever it’s necessary to see the protocol used in the experiment, either when the experiment is active or afterwords, e.g. when it is being written up, just click on the link and you are taken to the protocol. Links can also be made to the constructs used in the experiment, and even the strains. It’s also possible to link to a record showing where the strains or other samples used in the experiment are stored in the freezer, and also what has been done to them in the experiment.
Sharing with lab members
PI and postdoc
The article contains the following note explaining the division of labor between the two authors:
“Conceived and designed the experiments: GIL DB. Performed the experiments: GIL. Analyzed the data: GIL DB. Wrote the paper: GIL DB.”
So the paper notebook is Greg’s notebook. And, as the note says, he is the one who performed the experiments. Presumably David, Greg’s PI, commented and provided advice as the experiments were being carried out, but this is not reflected in the notebook narrative.
The electronic lab notebook opens up new possibilities for collaboration, and attribution. For example David could have been given permission to view, and edit, the experiment form, and could use the Discussion field for making his comments as the experiments were carried out, and these would be recorded as part of the experimental record. For example

David could do this at any time, because eCAT is a webapp and hence available 24/7. So he could review the experimental narrative not just when he’s looking at Greg’s paper notebook, and not just when he is in the lab; he could review and make comments from home or when travelling. So it’s much easier to communicate about the experiment. And the process is more transparent because there would be a dated record of David’s comments.
Bigger groups
That’s useful enough when just a postdoc and a PI are involved, as in this case, but it’s even more useful when a larger group is involved in working together on a series of experiments that are going to be written up in a paper. In that case the group can take full advantage of the electronic lab notebook’s linking and variable permissions capability by, say, having common access to shared resources like protocols and strains, and selective access to the experiment record, e.g. the key contributors have view and edit permission for the experiment record, and others have only view permission. Links can also be made to the publication in progress. And of course everyone can access all these records in the electronic notebook lab notebook 24/7; the physical and temporal limitations of the paper lab book are left behind.
Sharing with the community
New possibilities are also opened up for sharing with the community. Whether it is during the course of the experiment — for open science advocates — or after the experiment has been written up and published, having the experiment documented in the electronic lab notebook makes it much easier to share it with the interested community beyond the lab. And that sharing has far higher utility, because it allows the authors, and others, to search more effectively.
Search and archiving
It’s just not practical to search for terms, concepts, etc. in a 100+ page paper notebook. Not even for the author, much less for others like readers of the publication. Search is useful for those doing the experiment as they carry it out. E.g. in Greg’s case it would have been useful to search for ‘strain’ + ‘transformation’ + [a particular strain]‘. But these kinds of searches are also invaluable after the fact, both for the researcher and for those wanting to understand, recreate or build on the original research. So with the electronic lab notebook you get a shareable archive of the experimental results and the data associated with them.
Attribution
The electronic lab notebook also keeps an automatic record each time a change is made, so using its audit trial it’s possible to see who made what change when. This is useful in tracing the evolution of the experiment, and also in determining who contributed and in what way.
iPad electronic lab notebook: the best of both worlds
The paper lab notebook has one major advantage over an electronic lab notebook on a pc or mac– the paper lab notebook is portable. But electronic lab notebooks that work on mobile devices like the iPad are also portable, so that distinction also becomes a thing of the past. And the iPad opens up new possibilities for individual mobility and sharing with colleagues. So you get portability, just like the paper lab book; plus all the benefits noted above of the electronic lab notebook. It’s the best of both worlds!